THE POLYMORPHISM -308 G/A OF THE TNF GENE AND METABOLIC IMBALANCE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS AND NON-ALCOHOLIC FATTY LIVER DISEASE, TAKING INTO ACCOUNT CARDIOVASCULAR COMPLICATIONS

Non-alcoholic fatty liver disease (NAFLD), the most common form of disease, is now recognized as a major public health problem worldwide. Tumor necrosis factor alfa (TNF-α), member TNF/TNFR cytokine family, an intercellular transmission molecule that has been reported in wide range human noninfection diseases. The aim study was to determine circulating levels TNF-α and nature its relationships with components insulin resistance, both metabolic hormonal, patients type 2 diabetes; establishing cardiovascular complications (taking into account gene polymorphism) presence absence NAFLD. Materials methods. Case-control included information about 50 practically healthy people from city Kharkiv region. examined population (except control subjects) consisted exclusively diabetes mellitus long-term existence against background syndrome, varying degrees glycemic violations homeostasis renal failure. 117 were selected for analysis: 63 them NAFLD 54 without Genotyping single nucleotide polymorphism -308 G>A performed by method polymerase chain reaction appropriate primers NcoI endonuclease. Testing statistical hypotheses carried out using odds ratio χ2 criteria at significance level P≤0.05. Results. contribution genetic component formation predisposition development based on single-nucleotide TNFα determined, which makes it possible consider carrier A allele increased risk mellitus. No association studied developing found. obtained data make assume more associated diabetes, occurrence or progression primarily depends imbalance, not polymorphism. Conclusions. closely related hormonal factors markers may increase progressing complications.